PRNP

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA042754

 

Antibody HPA043398

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA042754 HPA043398 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

9 13 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

77 64 

Antigen sequence

SDYEDRYYRENMHRYPNQVYYRPMDEYSNQNNFVHDCVNITIKQHTVTTT
TKGENFTETDVKMMERVVEQMCITQYE
 
GSPGGNRYPPQGGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQ
GGGTHSQWNKPSKP
 

Matching transcripts

PRNP-002 - ENSP00000368752 [100%]
PRNP-003 - ENSP00000399376 [100%]
PRNP-004 - ENSP00000411599 [100%]
PRNP-001 - ENSP00000415284 [97%]
 PRNP-001 - ENSP00000415284 [100%]
PRNP-002 - ENSP00000368752 [100%]
PRNP-003 - ENSP00000399376 [100%]
PRNP-004 - ENSP00000411599 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human lateral ventricle shows distinct cytoplasmic positivity in neuronal cells.
More information
 Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:200 1:500 

Literature conformity

Consistent with extensive gene/protein characterization data Consistent with extensive gene/protein characterization data 

RNA consistency

Mainly consistent with RNA expression data Mainly not consistent with RNA expression data 

         Immunofluorescence

Image

  

Description

Application not done for this antibody. Immunofluorescent staining of human cell line U-2 OS shows positivity in nuclear membrane & cytoplasm.
More information
 

Antibody dilution

 1:21 

Validation IF

 Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. 

         Western Blot

Image

  

Description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY421589)
More information
 

Target mass (kDa)

27.7, 27.3, 23.6 27.7, 27.3, 23.6 

Antibody dilution

1:125 1:125 

Validation WB

Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present Uncertain: No bands detected 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:500 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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PRNP-001
 
PRNP-002
 
PRNP-003
 
PRNP-004