PTTG1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA008890

 

Antibody HPA045034

 

Antibody CAB008373

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 MBL
 

Product name

HPA008890 HPA045034 K0090-3 

Host species

Rabbit Rabbit Mouse 

Clonality

pAb pAb mAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Not known 

Released in version

3 13 3 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment Recombinant protein 

Length (aa)

132 45  

Antigen sequence

PPALPKATRKALGTVNRATEKSVKTKGPLKQKQPSFSAKKMTEKTVKAKS
SVPASDDAYPEIEKFFPFNPLDFESFDLPEEHQIAHLPLSGVPLMILDEE
RELEKLFQLGPPSPVKMPSPPWESNLLQSPSS
 
LPKATRKALGTVNRATEKSVKTKGPLKQKQPSFSAKKMTEKTVKA
 
 

Matching transcripts

PTTG1-001 - ENSP00000377536 [100%]
PTTG1-002 - ENSP00000344936 [100%]
PTTG1-006 - ENSP00000430642 [100%]
 PTTG1-001 - ENSP00000377536 [100%]
PTTG1-002 - ENSP00000344936 [100%]
PTTG1-004 - ENSP00000431068 [100%]
PTTG1-006 - ENSP00000430642 [100%]
  

Other gene match

PTTG2 - ENSG00000250254 [89%]
 PTTG2 - ENSG00000250254 [93%]
  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.
More information
 Immunohistochemical staining of human testis shows strong cytoplasmic positivity in a subset of seminiferus ducts.
More information
 Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.
More information
 

Retrieval method

HIER pH6 HIER pH6 HIER pH6 

Antibody dilution

1:100 1:450 1:100 

Literature conformity

Consistent with extensive gene/protein characterization data Consistent with extensive gene/protein characterization data Consistent with extensive gene/protein characterization data 

RNA consistency

Mainly consistent with RNA expression data Mainly consistent with RNA expression data No internal RNA expression data available for correlation 

         Western Blot

Image

   

Description

  Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

22, 21.1 22, 21.1, 12.9 22, 12.9 

Antibody dilution

1:250 1:250 1:500 

Validation WB

Non-supportive: Only bands not corresponding to the predicted size Non-supportive: Only bands not corresponding to the predicted size Uncertain: Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data 

         Protein array

Image

   

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. 

Antibody dilution

1:3000 1:6000  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen.  
 

ANTIGEN VIEW

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PTTG1-001
 
PTTG1-002
 
PTTG1-004
 
PTTG1-006