MADD

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA038568

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA038568 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

8 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

98 

Antigen sequence

YSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFM
EVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQ
 

Matching transcripts

MADD-001 - ENSP00000343902 [100%]
MADD-002 - ENSP00000385585 [100%]
MADD-003 - ENSP00000384435 [100%]
MADD-004 - ENSP00000378745 [100%]
MADD-005 - ENSP00000304505 [100%]
MADD-006 - ENSP00000310933 [100%]
MADD-007 - ENSP00000384204 [100%]
MADD-009 - ENSP00000384287 [100%]
MADD-018 - ENSP00000384483 [100%]
MADD-023 - ENSP00000378753 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human stomach, lower shows moderate cytoplasmic and membranous positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:1200 

Literature conformity

Partly consistent with extensive gene/protein characterization data 

RNA consistency

Not done 

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane & cytoplasm.
More information
 

Antibody dilution

1:200 

Validation IF

Supportive: The subcellular location is supported by experimental gene/protein characterization data, gene silencing, or an independent antibody. 

         Western Blot

Image

 

Description

 

Target mass (kDa)

183.3, 178.6, 176.6, 176.5, 175.9, 173.4, 171.4, 171, 164, 49.1 

Antibody dilution

1:250 

Validation WB

Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:60000 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 
 

ANTIGEN VIEW

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MADD-001
 
MADD-002
 
MADD-003
 
MADD-004
 
MADD-005
 
MADD-006
 
MADD-007
 
MADD-009
 
MADD-018
 
MADD-023