ZNF850

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA029299

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA029299 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

12 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

77 

Antigen sequence

VMFQDLSIDFSQEEWECLDAAQKDLYRDVMMENYSSLVSLGLSIPKPDVI
SLLEQGKEPWMVSRDVLGGWCRDERPH
 

Matching transcripts

ZNF850-001 - ENSP00000464976 [95%]
ZNF850-002 - ENSP00000465189 [95%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human placenta shows distinct positivity in subsets of cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:350 

Literature conformity

No avaliable gene/protein characterization data 

RNA consistency

Mainly consistent with RNA expression data 

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line RH-30 shows positivity in nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

1:78 

Validation IF

Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. 

         Western Blot

Image

 

Description

 

Target mass (kDa)

125.4, 9.7 

Antibody dilution

1:250 

Validation WB

Non-supportive: Only bands not corresponding to the predicted size 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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ZNF850-001
 
ZNF850-002