NEU4

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA037394

 

Antibody HPA037395

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA037394 HPA037395 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

12 7 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

94 88 

Antigen sequence

PRDDSWSVGPRSPLQPPLLGPGVHEPPEEAAVDPRGGQVPGGPFSRLQPR
GDGPRQPGPRPGVSGDVGSWTLALPMPFAAPPQSPTWLLYSHPV
 
RRARLHMGIRLSQSPLDPRSWTEPWVIYEGPSGYSDLASIGPAPEGGLVF
ACLYESGARTSYDEISFCTFSLREVLENVPASPKPPNL
 

Matching transcripts

NEU4-001 - ENSP00000385149 [99%]
NEU4-002 - ENSP00000375830 [99%]
NEU4-003 - ENSP00000320318 [99%]
NEU4-004 - ENSP00000384804 [99%]
NEU4-007 - ENSP00000385402 [99%]
 NEU4-001 - ENSP00000385149 [100%]
NEU4-002 - ENSP00000375830 [100%]
NEU4-003 - ENSP00000320318 [100%]
NEU4-004 - ENSP00000384804 [100%]
NEU4-007 - ENSP00000385402 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human colon shows strong cytoplasmic positivity in endocrine cells.
More information
 Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:35 1:60 

Literature conformity

Partly consistent with gene/protein characterization data Partly consistent with gene/protein characterization data 

RNA consistency

Mainly not consistent with RNA expression data Mainly not consistent with RNA expression data 

         Western Blot

Image

  

Description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY409058)
More information
 

Target mass (kDa)

53.1, 52.9, 51.6 53.1, 52.9, 51.6 

Antibody dilution

1:250 1:250 

Validation WB

Current setup is not applicable due to low RNA count Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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NEU4-001
 
NEU4-002
 
NEU4-003
 
NEU4-004
 
NEU4-007