SEPT5

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA029095

 

Antibody HPA063885

 

Antibody CAB016120

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 Santa Cruz Biotechnology
 

Product name

HPA029095 HPA063885 sc-20040 

Host species

Rabbit Rabbit Mouse 

Clonality

pAb pAb mAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Protein A/G 

Released in version

14 14 4 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment Not known 

Length (aa)

52 38  

Antigen sequence

QQMTSKLTQDSRMESPIPILPLPTPDAETEKLIRMKDEELRRMQEMLQRM
KQ
 
VPLIAKADCLVPSEIRKLKERIREEIDKFGIHVYQFPE
 
 

Matching transcripts

SEPT5-001 - ENSP00000391311 [100%]
SEPT5-004 - ENSP00000372515 [100%]
 SEPT5-001 - ENSP00000391311 [100%]
SEPT5-002 - ENSP00000394541 [100%]
SEPT5-004 - ENSP00000372515 [100%]
SEPT5-008 - ENSP00000408678 [100%]
SEPT5-009 - ENSP00000378541 [100%]
SEPT5-010 - ENSP00000414488 [100%]
SEPT5-014 - ENSP00000384535 [100%]
  

Other gene match

   

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Immunohistochemical staining of human cerebellum shows distinct cytoplasmic positivity in Purkinje cells.
More information
 Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil.
More information
 Immunohistochemical staining of human cerebral cortex shows strong positivity in neuropil.
More information
 

Retrieval method

HIER pH6 HIER pH6 HIER pH6 

Antibody dilution

1:3500 1:25 1:1200 

Literature conformity

Consistent with gene/protein characterization data Consistent with gene/protein characterization data Consistent with gene/protein characterization data 

RNA consistency

Mainly consistent with RNA expression data Mainly consistent with RNA expression data Mainly consistent with RNA expression data 

         Immunofluorescence

Image

   

Description

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm, actin filaments & nucleus but excluded from the nucleoli.
More information
 Application not done for this antibody. Application not done for this antibody. 

Antibody dilution

1:94   

Validation IF

Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available.   

         Western Blot

Image

   

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
  Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

43.8, 42.8 43.8, 42.8, 39.3, 38.3, 34.7, 33.8, 20.6 43.8, 42.8, 39.3, 38.3, 34.7, 33.8, 20.6, 20.2, 12.5 

Antibody dilution

1:250 1:80 1:500 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Non-supportive: Only bands not corresponding to the predicted size Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

   

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. 

Antibody dilution

1:3000 1:550  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen.  
 

ANTIGEN VIEW

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SEPT5-001
 
SEPT5-002
 
SEPT5-004
 
SEPT5-008
 
SEPT5-009
 
SEPT5-010
 
SEPT5-014