For the genes where the mouse and human genes are orthologues, the mouse cell line NIH 3T3, is also stained. An overview of the subcellular location for the corresponding protein, targeted by the antibody/antibodies for this gene, is given by the subcellular location page. The main subcellular location of the encoded proteins and any additional locations are reported as well as staining characteristics, staining intensity and validation score. Additional locations are characterized by a markedly lower staining intensity than the main location. The assay is described here.
Three different organelle probes are displayed as different channels in the multicolor images - nuclear stain in blue, micro-tubules in red and ER in yellow. The antibody staining is shown in green. By using the "toggle channels"-buttons, the different channels can be turned on and off. The intensity toggle shows the pixel intensity range in 16 different colors, for the selected channel, antibody, nucleus, microtubules or ER:
To change which images to compare, drag and drop a miniature image from the bottom on one of the top two positions. Two images can be compared at a time.
All images are clickable for an enlarged view. The selected image will appear in large size and miniature images with all other staining results for this gene will be listed at the top left of the image. The selected miniature image has a green overlay.