OXR1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA027375

 

Antibody HPA027380

 

Antibody HPA027395

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA027375 HPA027380 HPA027395 

Host species

Rabbit Rabbit Rabbit 

Clonality

pAb pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

6 6 6 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment Recombinant protein fragment 

Length (aa)

77 93 93 

Antigen sequence

QDSFLHENSLHQEESQKENMPCGETAEFKQKQSVNKGKQGKEQNQDSQTE
AEELRKLWKTHTMQQTKQQRENIQQVS
 
PIREELVSSDELRQDKSSGASSESVQTVNQAEVESLTVKSESTGTPGHLR
SDTEHSTNEVGTLCHKTDLNNLEMAIKEDQIADNFQGISGPKE
 
EEYGIMCPMEEVMSAAMYKEILDSKIKESLPIDIDQLSGRDFCHSKKMTG
SNTEEIDSRIRDAGNDSASTAPRSTEESLSEDVFTESELSPIR
 

Matching transcripts

OXR1-001 - ENSP00000311026 [100%]
OXR1-002 - ENSP00000431014 [100%]
OXR1-006 - ENSP00000429205 [100%]
OXR1-012 - ENSP00000431966 [100%]
OXR1-018 - ENSP00000430701 [100%]
OXR1-019 - ENSP00000405424 [100%]
OXR1-201 - ENSP00000402918 [100%]
OXR1-202 - ENSP00000395032 [100%]
 OXR1-001 - ENSP00000311026 [100%]
OXR1-002 - ENSP00000431014 [100%]
OXR1-006 - ENSP00000429205 [100%]
OXR1-012 - ENSP00000431966 [100%]
OXR1-018 - ENSP00000430701 [100%]
OXR1-019 - ENSP00000405424 [100%]
OXR1-201 - ENSP00000402918 [100%]
OXR1-202 - ENSP00000395032 [100%]
 OXR1-001 - ENSP00000311026 [100%]
OXR1-002 - ENSP00000431014 [100%]
OXR1-006 - ENSP00000429205 [100%]
OXR1-012 - ENSP00000431966 [100%]
OXR1-019 - ENSP00000405424 [100%]
OXR1-201 - ENSP00000402918 [100%]
OXR1-202 - ENSP00000395032 [100%]
 

Other gene match

   

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Immunohistochemical staining of human epididymis shows strong positivity in glandular cells.
More information
 Immunohistochemical staining of human uterine corpus shows strong cytoplasmic positivity in glandular cells.
More information
 Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells and distinct staining in neuropil.
More information
 

Retrieval method

HIER pH6 HIER pH6 HIER pH6 

Antibody dilution

1:550 1:75 1:100 

Literature conformity

Consistent with gene/protein characterization data Consistent with gene/protein characterization data Consistent with gene/protein characterization data 

RNA consistency

Mainly consistent with RNA expression data Mainly consistent with RNA expression data Mainly consistent with RNA expression data 

         Immunofluorescence

Image

   

Description

Application not done for this antibody. Immunofluorescent staining of human cell line U-2 OS shows positivity in vesicles.
More information
 Immunofluorescent staining of human cell line A-431 shows positivity in vesicles & nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

 1:20 1:50 

Validation IF

 Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. 

         Western Blot

Image

   

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY405747)
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY405747)
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY405747)
More information
 

Target mass (kDa)

98, 97.9, 94.7, 93.7, 58.4, 55.7 98, 97.9, 94.7, 93.7, 58.4, 55.7 98, 97.9, 94.7, 93.7, 55.7 

Antibody dilution

1:250 1:125 1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

   

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:3000 1:3000 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). Supportive: Pass with single peak corresponding to interaction only with its own antigen. Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 
 

ANTIGEN VIEW

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OXR1-001
 
OXR1-002
 
OXR1-006
 
OXR1-012
 
OXR1-018
 
OXR1-019
 
OXR1-201
 
OXR1-202