TKT

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA029480

 

Antibody HPA029481

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA029480 HPA029481 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

6 6 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

62 85 

Antigen sequence

HYYEGGIGEAVSSAVVGEPGITVTHLAVNRVPRSGKPAELLKMFGIDRDA
IAQAVRGLITKA
 
IAKTFKGRGITGVEDKESWHGKPLPKNMAEQIIQEIYSQIQSKKKILATP
PQEDAPSVDIANIRMPSLPSYKVGDKIATRKAYGQ
 

Matching transcripts

TKT-001 - ENSP00000417773 [100%]
TKT-004 - ENSP00000391481 [100%]
TKT-007 - ENSP00000405455 [100%]
TKT-201 - ENSP00000296289 [100%]
 TKT-001 - ENSP00000417773 [100%]
TKT-004 - ENSP00000391481 [100%]
TKT-007 - ENSP00000405455 [100%]
TKT-201 - ENSP00000296289 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human kidney shows strong nuclear positivity in cells of tubules.
More information
 Immunohistochemical staining of human kidney shows distinct nuclear positivity in tubular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:9000 1:1500 

Literature conformity

No avaliable gene/protein characterization data No avaliable gene/protein characterization data 

RNA consistency

Not done Not done 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but excluded from the nucleoli.
More information
 Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

1:100 1:20 

Validation IF

Supportive: The subcellular location is supported by experimental gene/protein characterization data, gene silencing, or an independent antibody. Supportive: The subcellular location is supported by experimental gene/protein characterization data, gene silencing, or an independent antibody. 

         siRNA

Image

  

Description

Application not done for this antibody. Signal downregulation > 25% by both siRNA:s.
More information
 

Antibody dilution

 1:37 

Validation siRNA

 Supportive: The siRNA validation is supportive. 

         Western blot - siRNA

Image

  

Description

Application not done for this antibody. Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled
More information
 

Target mass (kDa)

 68.8, 67.9, 49.9 

Loading control

 Total protein image 

Antibody dilution

 1:82 

Validation WB-siRNA

 Supportive: Downregulation visible in both siRNA lanes 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

68.8, 67.9, 49.9 68.8, 67.9, 49.9 

Antibody dilution

1:250 1:250 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:500 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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TKT-001
 
TKT-004
 
TKT-007
 
TKT-201