ZFYVE28

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA038175

 

Antibody HPA057587

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA038175 HPA057587 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

7 14 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

76 99 

Antigen sequence

GMKLSATVIFNPKSPTSLDSAVATQEAASEPVAEGMDGGPHKLSTGATNC
LLHSCVCCGSCGDSREDVVERLREKC
 
ARFYYADEELNQVAAELDSLDGRKDPQRCTLLVSQFRSCQDNVLNIINQI
MDECIPQDRAPRDFCVKFPEEIRHDNLAGQLWFGAECLAAGSIIMNREL
 

Matching transcripts

ZFYVE28-001 - ENSP00000290974 [100%]
ZFYVE28-002 - ENSP00000425706 [100%]
ZFYVE28-006 - ENSP00000426299 [100%]
 ZFYVE28-001 - ENSP00000290974 [100%]
ZFYVE28-002 - ENSP00000425706 [100%]
ZFYVE28-003 - ENSP00000423694 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human placenta shows moderate membranous positivity in trophoblastic cells.
More information
 Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:400 1:800 

Literature conformity

Partly consistent with gene/protein characterization data Partly consistent with gene/protein characterization data 

RNA consistency

Mainly not consistent with RNA expression data Mainly consistent with RNA expression data 

         Immunofluorescence

Image

  

Description

Application not done for this antibody. Immunofluorescent staining of human cell line HeLa shows positivity in nucleus, plasma membrane & cytoplasm.
More information
 

Antibody dilution

 1:81 

Validation IF

 Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
  

Target mass (kDa)

96.5, 93, 88.1 96.5, 93, 32.7 

Antibody dilution

1:250 1:270 

Validation WB

Uncertain: No bands detected Non-supportive: Only bands not corresponding to the predicted size 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:3250 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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ZFYVE28-001
 
ZFYVE28-002
 
ZFYVE28-003
 
ZFYVE28-006