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ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA007043

 

Antibody HPA007044

 

Antibody HPA011956

 

Antibody CAB010052

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 Santa Cruz Biotechnology
 

Product name

HPA007043 HPA007044 HPA011956 sc-46695 

Host species

Rabbit Rabbit Rabbit Mouse 

Clonality

pAb pAb pAb mAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Protein A/G 

Released in version

5 3 4 3 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment Recombinant protein fragment Recombinant protein 

Length (aa)

136 127 94  

Antigen sequence

VSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKNK
EGLHNAKEILTRLGVEPSDDDCVSVQHVCTIVSFRSANLVAATLGAILNR
LRDNKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLR
 
LPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVHMESEVYD
TPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPVGFTFSFPCQQSKI
DEAILITWTKRFKASGVEGADVVKLLN
 
QGTGEELFDHIVQCIADFLDYMGLKGASLPLGFTFSFPCRQMSIDKGTLI
GWTKGFKATDCEGEDVVDMLREAIKRRNEFDLDIVAVVNDTVGT
 
 

Matching transcripts

HK1-001 - ENSP00000352398 [100%]
HK1-201 - ENSP00000298649 [100%]
HK1-202 - ENSP00000353433 [100%]
HK1-203 - ENSP00000402103 [100%]
 HK1-001 - ENSP00000352398 [100%]
HK1-016 - ENSP00000415949 [100%]
HK1-201 - ENSP00000298649 [100%]
HK1-202 - ENSP00000353433 [100%]
HK1-203 - ENSP00000402103 [100%]
 HK1-001 - ENSP00000352398 [80%]
HK1-201 - ENSP00000298649 [80%]
HK1-202 - ENSP00000353433 [80%]
HK1-203 - ENSP00000402103 [80%]
  

Other gene match

  HKDC1 - ENSG00000156510 [100%]
  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

    

Description

Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
More information
 Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in parietal cells.
More information
 Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in granular pattern in neuronal cells.
More information
 Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 HIER pH6 HIER pH6 

Antibody dilution

1:100 1:15 1:150 1:600 

Literature conformity

Consistent with extensive gene/protein characterization data Consistent with extensive gene/protein characterization data Consistent with extensive gene/protein characterization data Consistent with extensive gene/protein characterization data 

RNA consistency

Not done Not done Not done Not done 

         Immunofluorescence

Image

    

Description

Application not done for this antibody. Immunofluorescent staining of human cell line U-2 OS shows positivity in mitochondria.
More information
 Application not done for this antibody. Immunofluorescent staining of human cell line A-431 shows positivity in mitochondria.
More information
 

Antibody dilution

 1:5  1:150 

Validation IF

 Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available.  Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. 

         Western Blot

Image

    

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

102.7, 102.5, 102.2, 101.1 102.7, 102.5, 102.2, 101.1, 32.7 102.7, 102.5, 102.2, 101.1 102.7, 102.5, 102.2, 101.1, 32.7, 15.7, 13 

Antibody dilution

1:250 1:250 1:250 1:500 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) Supportive: Single band corresponding to the predicted size in kDa (+/-20%) Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

    

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. 

Antibody dilution

1:3000 1:500 1:3000  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). Supportive: Pass with single peak corresponding to interaction only with its own antigen.  
 

ANTIGEN VIEW

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HK1-001
 
HK1-016
 
HK1-201
 
HK1-202
 
HK1-203