EEF1A1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA051759

 

Antibody HPA053862

 

Antibody HPA056990

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA051759 HPA053862 HPA056990 

Host species

Rabbit Rabbit Rabbit 

Clonality

pAb pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

12 12 12 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment Recombinant protein fragment 

Length (aa)

45 32 49 

Antigen sequence

LLEALDTILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGIL
 
PVIDCHTAHIACKFAELKEKIDRRSGKKLEDN
 
PGMVVTFAPVNITTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDIRR
 

Matching transcripts

EEF1A1-001 - ENSP00000339063 [96%]
EEF1A1-004 - ENSP00000330054 [96%]
EEF1A1-009 - ENSP00000339053 [96%]
EEF1A1-201 - ENSP00000479609 [96%]
EEF1A1-202 - ENSP00000479055 [80%]
 EEF1A1-001 - ENSP00000339063 [94%]
EEF1A1-004 - ENSP00000330054 [94%]
EEF1A1-009 - ENSP00000339053 [94%]
EEF1A1-201 - ENSP00000479609 [94%]
EEF1A1-202 - ENSP00000479055 [94%]
 EEF1A1-001 - ENSP00000339063 [96%]
EEF1A1-004 - ENSP00000330054 [96%]
EEF1A1-009 - ENSP00000339053 [96%]
EEF1A1-201 - ENSP00000479609 [96%]
EEF1A1-202 - ENSP00000479055 [96%]
 

Other gene match

EEF1A2 - ENSG00000101210 [100%]
 EEF1A2 - ENSG00000101210 [100%]
 EEF1A2 - ENSG00000101210 [100%]
 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
More information
 Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
More information
 Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 HIER pH6 

Antibody dilution

1:50 1:200 1:1000 

Literature conformity

Consistent with gene/protein characterization data Partly consistent with gene/protein characterization data Partly consistent with gene/protein characterization data 

RNA consistency

Not done Not done Not done 

         Immunofluorescence

Image

   

Description

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
More information
 Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
More information
 Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
More information
 

Antibody dilution

1:28 1:23 1:103 

Validation IF

Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. 

         Western Blot

Image

   

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

50.5, 50.1, 47.9, 46.4 50.5, 50.1, 47.9, 46.4 50.5, 50.1, 47.9, 46.4 

Antibody dilution

1:140 1:120 1:340 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Single band corresponding to the predicted size in kDa (+/-20%) Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

   

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:1100 1:950 1:4100 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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EEF1A1-001
 
EEF1A1-004
 
EEF1A1-009
 
EEF1A1-201
 
EEF1A1-202