GABRA6

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA019188

 

Antibody HPA058329

 

Antibody CAB022462

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 Chemicon
 

Product name

HPA019188 HPA058329 AB5610 

Host species

Rabbit Rabbit Rabbit 

Clonality

pAb pAb msAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Affinity 

Released in version

12 12 12 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment Synthetic peptide 

Length (aa)

89 24  

Antigen sequence

TNLQTQKAKRKAQFAAPPTVTISKATEPLEAEIVLHPDSKYHLKKRITSL
SLPIVSSSEANKVLTRAPILQSTPVTPPPLSPAFGGTSK
 
GKLEVEGNFYSENVSRILDNLLEG
 
 

Matching transcripts

GABRA6-001 - ENSP00000274545 [100%]
GABRA6-003 - ENSP00000430527 [100%]
 GABRA6-001 - ENSP00000274545 [100%]
GABRA6-003 - ENSP00000430527 [100%]
  

Other gene match

   

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
More information
 Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
More information
 Immunohistochemical staining of human cerebellum shows distinct positivity in neuropil.
More information
 

Retrieval method

HIER pH6 HIER pH6 HIER pH6 

Antibody dilution

1:40 1:125 1:175 

Literature conformity

No avaliable gene/protein characterization data No avaliable gene/protein characterization data No avaliable gene/protein characterization data 

RNA consistency

Not consistent with RNA expression data Not consistent with RNA expression data Not consistent with RNA expression data 

         Western Blot

Image

   

Description

   

Target mass (kDa)

51, 49.7 51, 49.7 51, 49.7, 22.6, 22, 21.2 

Antibody dilution

1:250 1:170 1:500 

Validation WB

Current setup is not applicable due to low RNA count Current setup is not applicable due to low RNA count Current setup is not applicable due to low RNA count 

         Protein array

Image

   

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. 

Antibody dilution

1:3000 1:2050  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen.  
 

ANTIGEN VIEW

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GABRA6-001
 
GABRA6-003