TMEM106C

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA047204

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA047204 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

10 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

30 

Antigen sequence

SVKISYIGLMTQSSLETHHYVDCGGNSTAI
 

Matching transcripts

TMEM106C-001 - ENSP00000400471 [100%]
TMEM106C-002 - ENSP00000256686 [100%]
TMEM106C-003 - ENSP00000402705 [100%]
TMEM106C-004 - ENSP00000446657 [100%]
TMEM106C-011 - ENSP00000448268 [100%]
TMEM106C-012 - ENSP00000449737 [100%]
TMEM106C-021 - ENSP00000448959 [83%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in renal tubules.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:35 

Literature conformity

Consistent with gene/protein characterization data 

RNA consistency

Mainly consistent with RNA expression data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

27.9, 25.8, 19.8, 12.1 

Antibody dilution

1:240 

Validation WB

Uncertain: Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:1950 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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TMEM106C-001
 
TMEM106C-002
 
TMEM106C-003
 
TMEM106C-004
 
TMEM106C-011
 
TMEM106C-012
 
TMEM106C-021