RALY

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA040971

 

Antibody HPA043614

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA040971 HPA043614 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

10 10 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

45 66 

Antigen sequence

QENTTSEAGLPQGEARTRDDGDEEGLLTHSEEELEHSQDTDADDG
 
RLFDYRGRLSPVPVPRAVPVKRPRVTVPLVRRVKTNVPVKLFARSTAVTT
SSAKIKLKSSELQAIK
 

Matching transcripts

RALY-001 - ENSP00000364255 [100%]
RALY-002 - ENSP00000246194 [100%]
 RALY-001 - ENSP00000364255 [100%]
RALY-002 - ENSP00000246194 [100%]
RALY-005 - ENSP00000327522 [100%]
RALY-007 - ENSP00000415973 [100%]
RALY-004 - ENSP00000413638 [85%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human cerebellum shows strong nuclear positivity in purkinje cells, cells in molecular layer and cells in granular layer.
More information
 Immunohistochemical staining of human kidney shows strong nuclear positivity in tubular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:1000 1:2500 

Literature conformity

Consistent with gene/protein characterization data Consistent with gene/protein characterization data 

RNA consistency

Not done Not done 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line U-2 OS shows positivity in vesicles & nucleus but excluded from the nucleoli.
More information
 Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

1:33 1:156 

Validation IF

Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. 

         Western blot - siRNA

Image

  

Description

Application not done for this antibody. Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled
More information
 

Target mass (kDa)

 32.5, 30.4, 24.7, 20.2, 18.7 

Loading control

 Total protein image 

Antibody dilution

 1:349 

Validation WB-siRNA

 Supportive: Downregulation visible in one of two siRNA lanes 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

32.5, 30.4 32.5, 30.4, 24.7, 20.2, 18.7 

Antibody dilution

1:250 1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:6000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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RALY-001
 
RALY-002
 
RALY-004
 
RALY-005
 
RALY-007