CKS2

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA030762

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA030762 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

7 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

76 

Antigen sequence

HKQIYYSDKYFDEHYEYRHVMLPRELSKQVPKTHLMSEEEWRRLGVQQSL
GWVHYMIHEPEPHILLFRRPLPKDQQ
 

Matching transcripts

CKS2-001 - ENSP00000364976 [100%]
 

Other gene match

CKS1B - ENSG00000173207 [82%]
 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human pancreas shows strong nuclear, cytoplasmic and membranous positivity in exocrine glandular cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:150 

Literature conformity

Partly consistent with gene/protein characterization data 

RNA consistency

Not done 

         Western Blot

Image

 

Description

Target too small/large to be analyzed with the present setup 

Target mass (kDa)

9.9, 9.7 

Antibody dilution

 

Validation WB

Target too small/large to be analyzed with the present setup 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:500 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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CKS2-001