EPM2A

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA053643

 

Antibody HPA055468

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA053643 HPA055468 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

11 11 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

115 78 

Antigen sequence

ATGHTNEMKHTTDFYFNIAGHQAMHYSRILPNIWLGSCPRQVEHVTIKLK
HELGITAVMNFQTEWDIVQNSSGCNRYPEPMTPDTMIKLYREEGLAYIWM
PTPDMSTEGRVQMLP
 
HALLEKGHIVYVHCNAGVGRSTAAVCGWLQYVMGWNLRKVQYFLMAKRPA
VYIDEEALARAQEDFFQKFGKVRSSVCS
 

Matching transcripts

EPM2A-002 - ENSP00000356489 [100%]
EPM2A-004 - ENSP00000405913 [100%]
EPM2A-202 - ENSP00000480339 [100%]
EPM2A-001 - ENSP00000414900 [94%]
EPM2A-201 - ENSP00000480268 [94%]
 EPM2A-002 - ENSP00000356489 [100%]
EPM2A-201 - ENSP00000480268 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human colon shows moderate cytoplasmic and nuclear positivity in glandular cells.
More information
 Immunohistochemical staining of human salivary gland shows strong cytoplasmic positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:25 1:30 

Literature conformity

Partly consistent with extensive gene/protein characterization data Partly consistent with extensive gene/protein characterization data 

RNA consistency

Not done Not done 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli.
More information
 Immunofluorescent staining of human cell line SH-SY5Y shows positivity in nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

1:33 1:5 

Validation IF

Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. 

         Western Blot

Image

  

Description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

37.2, 35.5, 27.1, 22.2, 19.5 37.2, 22.2 

Antibody dilution

1:160 1:80 

Validation WB

Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:1300 1:500 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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EPM2A-001
 
EPM2A-002
 
EPM2A-004
 
EPM2A-201
 
EPM2A-202