GPRC5D

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA071909

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA071909 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

14 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

26 

Antigen sequence

MYKDCIESTGDYFLLCDAEGPWGIIL
 

Matching transcripts

GPRC5D-001 - ENSP00000228887 [100%]
GPRC5D-002 - ENSP00000379624 [100%]
GPRC5D-003 - ENSP00000440530 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in islets of Langerhans.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:900 

Literature conformity

Consistent with gene/protein characterization data 

RNA consistency

Mainly not consistent with RNA expression data 

         Western Blot

Image

 

Description

 

Target mass (kDa)

38.8, 34.1, 13.4 

Antibody dilution

1:90 

Validation WB

Non-supportive: Only bands not corresponding to the predicted size 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:1000 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 
 

ANTIGEN VIEW

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GPRC5D-001
 
GPRC5D-002
 
GPRC5D-003