CHKA

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA024153

 

Antibody CAB072803

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 NCI-CPTC
 

Product name

HPA024153 CPTC-CHKA-1 

Host species

Rabbit Mouse 

Clonality

pAb mAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Protein A/G 

Released in version

5 14 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein 

Length (aa)

91  

Antigen sequence

FDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDF
ENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKIS
 
 

Matching transcripts

CHKA-001 - ENSP00000265689 [100%]
CHKA-002 - ENSP00000348454 [100%]
  

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferous ducts.
More information
 Application not done for this antibody. 

Retrieval method

HIER pH6  

Antibody dilution

1:15  

Literature conformity

Consistent with extensive gene/protein characterization data  

RNA consistency

Mainly not consistent with RNA expression data  

         Immunofluorescence

Image

  

Description

Application not done for this antibody. Immunofluorescent staining of human cell line U-2 OS shows positivity in endoplasmic reticulum.
More information
 

Antibody dilution

 1:6 

Validation IF

 Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403943)
More information
 Application not done for this antibody. 

Target mass (kDa)

52.2, 50.2  

Antibody dilution

1:250  

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%)  

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. 

Antibody dilution

1:3000  

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).  
 

ANTIGEN VIEW

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CHKA-001
 
CHKA-002