VSIG1

ANTIBODY AND ANTIGEN INFORMATION

? »
 

Antibody HPA001445

 

Antibody HPA036310

 

Antibody HPA036311

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA001445 HPA036310 HPA036311 

Host species

Rabbit Rabbit Rabbit 

Clonality

pAb pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

7 7 7 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment Recombinant protein fragment 

Length (aa)

107 60 66 

Antigen sequence

QAVAIGQFKDRITGSNDPGNASITISHMQPADSGIYICDVNNPPDFLGQN
QGILNVSVLVKPSKPLCSVQGRPETGHTISLSCLSALGTPSPVYYWHKLE
GRDIVPV
 
KAKAKAKERNSKTIAELEPMTKINPRGESEAMPREDATQLEVTLPSSIHE
TGPDTIQEPD
 
EPKPTQEPAPEPAPGSEPMAVPDLDIELELEPETQSELEPEPEPEPESEP
GVVVEPLSEDEKGVVK
 

Matching transcripts

VSIG1-001 - ENSP00000217957 [100%]
VSIG1-201 - ENSP00000402219 [100%]
 VSIG1-001 - ENSP00000217957 [100%]
VSIG1-201 - ENSP00000402219 [100%]
 VSIG1-001 - ENSP00000217957 [100%]
VSIG1-201 - ENSP00000402219 [100%]
 

Other gene match

   

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Immunohistochemical staining of human stomach shows strong membranous and cytoplasmic positivity in glandular cells.
More information
 Immunohistochemical staining of human stomach shows strong membranous and cytoplasmic positivity in glandular cells.
More information
 Immunohistochemical staining of human testis shows strong cytoplasmic and membranous positivity in cells in seminiferus ducts.
More information
 

Retrieval method

HIER pH6 HIER pH6 HIER pH6 

Antibody dilution

1:750 1:2000 1:4000 

Literature conformity

Consistent with gene/protein characterization data Consistent with gene/protein characterization data Consistent with gene/protein characterization data 

RNA consistency

Mainly consistent with RNA expression data Consistent with RNA expression data Mainly consistent with RNA expression data 

         Immunofluorescence

Image

   

Description

Application not done for this antibody. Immunofluorescent staining of human cell line PC-3 shows positivity in cytoplasm & vesicles.
More information
 Application not done for this antibody. 

Antibody dilution

 1:200  

Validation IF

 Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available.  

         Western Blot

Image

   

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403639)
More information
 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

45.8, 41.8 45.8, 41.8 45.8, 41.8 

Antibody dilution

1:250 1:250 1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

   

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:3000 1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 
 

ANTIGEN VIEW

? »
 
 
 
VSIG1-001
 
VSIG1-201