CEP192

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA039392

 

Antibody HPA040503

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA039392 HPA040503 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

7 7 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

92 87 

Antigen sequence

KGVDESGDVFRATYAAFRCSPISGLLESHGIQKVSITFLPRGRGDYAQFW
DVECHPLKEPHMKHTLRFQLSGQSIEAENEPENACLSTDSLI
 
FVLKERTQENVTLIYNPSDRGINNKTATELSTVYLFGGDEISRQQYRRAL
LHKPEMIKQILPEHSVLQNINFVEAFQDELLVTEVYD
 

Matching transcripts

CEP192-001 - ENSP00000427550 [100%]
CEP192-010 - ENSP00000467038 [100%]
CEP192-201 - ENSP00000317156 [100%]
 CEP192-001 - ENSP00000427550 [100%]
CEP192-010 - ENSP00000467038 [100%]
CEP192-201 - ENSP00000317156 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity.
More information
 Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear staining in seminiferus ducts.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:35 1:125 

Literature conformity

Partly consistent with gene/protein characterization data Partly consistent with gene/protein characterization data 

RNA consistency

Not done Not done 

         Immunofluorescence

Image

  

Description

Application not done for this antibody. Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm & centrosome.
More information
 

Antibody dilution

 1:71 

Validation IF

 Supportive: The subcellular location is supported by experimental gene/protein characterization data, gene silencing, or an independent antibody. 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

279.1, 228.4, 227 279.1, 228.4, 227 

Antibody dilution

1:250 1:250 

Validation WB

Uncertain: No bands detected Uncertain: Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:500 1:3000 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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CEP192-001
 
CEP192-010
 
CEP192-201