FAM72C

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA043271

 

Antibody HPA067321

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA043271 HPA067321 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

10 14 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

51 24 

Antigen sequence

CVSILCCKFCKQVLSSRGMKAVLLADTEIDLFSTDIPPTNAVDFTGRCYF
T
 
GNLPEIEESTDEDVLNISAEECIR
 

Matching transcripts

FAM72C-001 - ENSP00000463032 [100%]
FAM72C-003 - ENSP00000358174 [100%]
 FAM72C-001 - ENSP00000463032 [100%]
 

Other gene match

FAM72B - ENSG00000188610 [100%]
FAM72A - ENSG00000196550 [100%]
FAM72D - ENSG00000215784 [100%]
 FAM72B - ENSG00000188610 [100%]
FAM72A - ENSG00000196550 [100%]
FAM72D - ENSG00000215784 [100%]
 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human soft tissue shows moderate cytoplasmic positivity in peripheral nerve.
More information
 Application not done for this antibody. 

Retrieval method

HIER pH6  

Antibody dilution

1:600  

Literature conformity

No avaliable gene/protein characterization data  

RNA consistency

Not consistent with RNA expression data  

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm & vesicles.
More information
 Immunofluorescent staining of human cell line A-431 shows positivity in vesicles.
More information
 

Antibody dilution

1:103 1:60 

Validation IF

Supportive: The subcellular location is supported by experimental gene/protein characterization data, gene silencing, or an independent antibody. Non-supportive: The subcellular location is not consistent with experimental gene/protein characterization data, or conflicting with an independent antibody. 

         Western Blot

Image

  

Description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

16.7, 16.6, 10.8 16.7, 16.6, 16.4, 13.4, 12.2 

Antibody dilution

1:250 1:200 

Validation WB

Non-supportive: Only bands not corresponding to the predicted size Uncertain: Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:6000 1:2400 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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FAM72C-001
 
FAM72C-003