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GENERAL INFORMATION
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Protein class
Protein class the gene product belongs to according to selected gene lists. List of protein classes.
Predicted localization
Protein localization predicted from several bioinformatic algorithms. More info...
Number of transcripts
Number of protein-coding transcribed from this gene as defined by Ensembl.
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Gene name |
CYP3A4 |
Gene description |
Cytochrome P450, family 3, subfamily A, polypeptide 4 |
Protein class |
Cancer-related genes
Enzymes
Plasma proteins
Predicted intracellular proteins
Predicted membrane proteins |
Predicted localization |
Intracellular,Membrane |
Number of transcripts |
3 |
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HUMAN PROTEIN ATLAS INFORMATION
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RNA tissue category
The RNA tissue category is based on mRNA expression levels in 32 different tissues ( RNA assay description).
The categories include: tissue enriched, group enriched, tissue enhanced, expressed in all, not detected, and mixed.
RNA tissue category is calculated separately both for internally generated Human Protein Atlas (HPA) RNA-seq data, as
well as RNA-seq data from the Genotype-Tissue Expression ( GTEx) consortium.
Protein evidence
Protein evidence scores are generated from several independent sources
and are classified as evidence at i) protein level, ii) transcript level, iii) no evidence or iv) not available.
Protein localization
A summary of the overall protein expression pattern across the analyzed tissues. The summary is based on knowledge-based
annotation ( Annotation description). For genes pending
knowledge-based annotation, the protein localization refers to the antibody staining pattern.
"RNA-based expert annotation gave inconconclusive results" is shown for genes analyzed with a knowledge-based approach where
available RNA-seq and gene/protein characterization data has been evaluated as not sufficient in combination with
immunohistochemistry-data to yield a reliable estimation of the protein expression profile.
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RNA tissue category |
| HPA: | Group enriched (duodenum, liver, small intestine) |
| GTEx: | Group enriched (liver, small intestine) |
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Protein evidence |
Evidence at protein level |
Protein localization |
Selective cytoplasmic expression in liver, small intestine and duodenum. |
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ANTIBODY RELIABILITY
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Data reliability description
Standardized explanatory sentences with additional information required for full understanding of the
knowledge-based expression profile. Currently, not all genes have been analyzed by knowledge-based
annotation, indicated by "Pending RNA-based expert annotation", when applicable.
Data reliability
Data reliability score ( score description), divided into supportive (premium)
or uncertain (not premium), is based on consistency between the staining pattern of one antibody or several
antibodies with RNA-seq data and available gene/protein characterization data.
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Data reliability
description |
Antibody staining consistent with RNA expression data. |
Data reliability |
Supportive based on 1 antibody.
CAB033671
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PROTEIN EXPRESSION OVERVIEW
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Protein expression data is shown for each of the 44 tissues. Color-coding is based on 13 tissue groups, each consisting
of tissues with common functional features. Mouse-over function shows protein score for analyzed cell types found in a
selected tissue. To access image data click on tissue name or bar. Annotation of protein expression is described in
detail in Assays & annotation.
For genes with available protein data for which a knowledge-based annotation could not be performed, no protein
expression data is displayed in the protein expression data overview, however, all immunohistochemical images are
still available and the annotation data can be found under Primary data.
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GENE INFORMATION
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Gene information from Ensembl and Entrez, as well as links to available gene identifiers are displayed here. Information was retrieved from Ensembl if not indicated otherwise.
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Gene name |
CYP3A4 (HGNC Symbol) |
Synonyms |
CYP3A3 |
Description |
Cytochrome P450, family 3, subfamily A, polypeptide 4 (HGNC Symbol) |
Entrez gene summary |
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Feb 2011] |
Chromosome |
7 |
Cytoband |
q22.1 |
Chromosome location (bp) |
99756960 - 99784265 |
Protein evidence |
Evidence at protein level (all genes) |
Ensembl |
ENSG00000160868 (version 78.38) |
Entrez gene |
1576 |
UniProt |
P08684 (UniProt - Evidence at protein level) |
neXtProt |
NX_P08684 |
Antibodypedia |
CYP3A4 antibodies |
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PROTEIN BROWSER
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The protein browser displays the antigen location on the target protein(s) and the features of the target protein. The
tabs at the top of the protein view section can be used to switch between the different splice variants to which
an antigen has been mapped.
At the top of the view, the position of the antigen (identified by the corresponding HPA identifier) is shown as a green
bar. A yellow triangle on the bar indicates a <100% sequence identity to the protein target.
Under the antigens, the maximum percent sequence identity of the protein to all other proteins from other human genes is
displayed, using a sliding window of 10 aa residues (HsID 10) or 50 aa residues
(HsID 50) ( read more).
If a signal peptide is predicted by a majority of the signal peptide predictors SPOCTOPUS,
SignalP 4.0, and
Phobius (turquoise)
and/or transmembrane regions (orange) are predicted by MDM, these are displayed.
Low complexity regions are shown in yellow
and InterPro regions in green. Common (purple) and
unique (grey) regions between different splice variants of the gene are also displayed
( read more), and at the
bottom of the protein view is the protein scale.
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PROTEIN INFORMATION
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The protein information section displays alternative protein-coding transcripts (splice variants) encoded by this
gene according to the Ensembl database.
The ENSP identifier links to the Ensembl website protein summary, while the ENST identifier links to the Ensembl website transcript summary
for the selected splice variant. The data in the UniProt column can be expanded to show links to all matching
UniProt identifiers for this protein.
The protein classes assigned to this protein are shown if expanding the data in the
protein class column. Parent protein classes are in bold font and subclasses are listed under the parent class.
The Gene Ontology terms assigned to this protein are
listed if expanding the Gene ontology column.
The length of the protein (amino acid residues according to Ensembl), molecular mass (kDalton), predicted signal peptide (according to a
majority of the signal peptide predictors SPOCTOPUS,
SignalP 4.0 and
Phobius) and the number of predicted transmembrane region(s)
(according to MDM) are also reported.
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Splice variant |
Ensembl |
UniProt |
Protein class |
Gene ontology |
Length
(aa) |
Molecular mass
(kDa) |
Signal peptide
(predicted) |
Transmembrane regions
(predicted)
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| CYP3A4-001 |
ENSP00000337915
ENST00000336411
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P08684
Show all »
« Hide
P08684 [Direct mapping]Cytochrome P450 3A4 Q6GRK0 [Target identity:100%; Query identity:100%]Cytochrome P450 family 3 subfamily A polypeptide 4 {ECO:0000313|EMBL:ABI96208.1}; Cytochrome P450, family 3, subfamily A, polypeptide 4 {ECO:0000313|EMBL:AAH69418.1}; Cytochrome P450, family 3, subfamily A, polypeptide 4, isoform CRA_a {ECO:0000313|EMBL:EAW76635.1}; Cytochrome P450, subfamily IIIA, polypeptide 4 {ECO:0000313|EMBL:AAI01632.1}; cDNA, FLJ93427, Homo sapiens cytochrome P450, family 3, subfamily A, polypeptide 4(CYP3A4), transcript variant 1, mRNA {ECO:0000313|EMBL:BAG35806.1} Q7Z448 [Target identity:85%; Query identity:100%]Cytochrome P450 {ECO:0000313|EMBL:CAD91645.1} B4DPQ5 [Target identity:64%; Query identity:100%]cDNA FLJ51521, highly similar to Cytochrome P450 3A4 {ECO:0000313|EMBL:BAG60667.1} Q9BZM0 [Target identity:9%; Query identity:100%]Cytochrome P450 CYP3A4 {ECO:0000313|EMBL:AAG53948.1}
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Enzymes
ENZYME proteins
Oxidoreductases
MEMSAT3 predicted membrane proteins
MEMSAT-SVM predicted membrane proteins
Phobius predicted membrane proteins
SPOCTOPUS predicted membrane proteins
THUMBUP predicted membrane proteins
TMHMM predicted membrane proteins
Phobius predicted secreted proteins
Predicted intracellular proteins
Plasma proteins
Cancer-related genes
Candidate cancer biomarkers
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
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503 |
57.3 |
No |
0 |
| CYP3A4-003 |
ENSP00000397208
ENST00000415003
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C9JBD2
Show all »
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C9JBD2 [Target identity:100%; Query identity:100%]Cytochrome P450 3A4 {ECO:0000313|Ensembl:ENSP00000397208}
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MEMSAT3 predicted membrane proteins
MEMSAT-SVM predicted membrane proteins
SPOCTOPUS predicted membrane proteins
THUMBUP predicted membrane proteins
Phobius predicted secreted proteins
Predicted intracellular proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
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GO:0005506 [iron ion binding] GO:0016705 [oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen] GO:0020037 [heme binding] GO:0055114 [oxidation-reduction process]
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153 |
17.3 |
No |
0 |
| CYP3A4-201 |
ENSP00000346607
ENST00000354593
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E7EVM8
Show all »
« Hide
E7EVM8 [Target identity:100%; Query identity:100%]Cytochrome P450 3A4 {ECO:0000313|Ensembl:ENSP00000346607} B4DPQ5 [Target identity:91%; Query identity:100%]cDNA FLJ51521, highly similar to Cytochrome P450 3A4 {ECO:0000313|EMBL:BAG60667.1} Q9BZM0 [Target identity:13%; Query identity:100%]Cytochrome P450 CYP3A4 {ECO:0000313|EMBL:AAG53948.1}
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Predicted membrane proteins
Prediction method-based
Membrane proteins predicted by MDM
MEMSAT3 predicted membrane proteins
MEMSAT-SVM predicted membrane proteins
Phobius predicted membrane proteins
SPOCTOPUS predicted membrane proteins
THUMBUP predicted membrane proteins
TMHMM predicted membrane proteins
# TM segments-based
3TM proteins predicted by MDM
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
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353 |
40.4 |
No |
3 |
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