FRMD6

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA001297

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA001297 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

1 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

135 

Antigen sequence

LEEDLQDDEIEMLVDDPRDLEQMNEESLEVSPDMCIYITEDMLMSRKLNG
HSGLIVKEIGSSTSSSSETVVKLRGQSTDSLPQTICRKPKTSTDRHSLSL
DDIRLYQKDFLRIAGLCQDTAQSYTFGCGHELDEE
 

Matching transcripts

FRMD6-001 - ENSP00000379068 [100%]
FRMD6-002 - ENSP00000343899 [100%]
FRMD6-003 - ENSP00000348550 [100%]
FRMD6-005 - ENSP00000452529 [100%]
FRMD6-013 - ENSP00000451977 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Mouse brain

Image

 

Description

Cytoplasmic and nuclear positivity in neurons in most areas of the brain, most abundant in olfactory bulb, selected areas of the cerebral cortex as well as ventricle wall choroid plexus.
More information
 

Validation MB

Uncertain 

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:500 

Literature conformity

No avaliable gene/protein characterization data 

RNA consistency

Not done 

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line U-2 OS shows positivity in mitochondria.
More information
 

Antibody dilution

1:80 

Validation IF

Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: EFO-21
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

72, 71, 62.9, 29.2 

Antibody dilution

1:500 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:1000 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 
 

ANTIGEN VIEW

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FRMD6-001
 
FRMD6-002
 
FRMD6-003
 
FRMD6-005
 
FRMD6-013