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Antibody HPA022434
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Antibody HPA022953
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Antibody HPA022959
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Antibody HPA028758
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Antibody CAB007783
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ANTIBODY INFORMATION
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Provider |
Atlas Antibodies Sigma-Aldrich
| | Atlas Antibodies Sigma-Aldrich
| | Atlas Antibodies Sigma-Aldrich
| | Atlas Antibodies Sigma-Aldrich
| | Epitomics
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Product name |
HPA022434 | | HPA022953 | | HPA022959 | | HPA028758 | | 1699-1 | |
Host species |
Rabbit | | Rabbit | | Rabbit | | Rabbit | | Rabbit | |
Clonality |
pAb | | pAb | | pAb | | pAb | | mAb | |
Purity |
Affinity purified using the PrEST-antigen as affinity ligand | | Affinity purified using the PrEST-antigen as affinity ligand | | Affinity purified using the PrEST-antigen as affinity ligand | | Affinity purified using the PrEST-antigen as affinity ligand | | Supernatant | |
Released in version |
5 | | 5 | | 5 | | 6 | | 3 | |
ANTIGEN INFORMATION
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Antigen |
Recombinant protein fragment | | Recombinant protein fragment | | Recombinant protein fragment | | Recombinant protein fragment | | Synthetic peptide | |
Length (aa) |
97 | | 98 | | 101 | | 90 | | | |
Antigen sequence |
AEEFYVCIYATREGDYVLFHHEGGVDVGDVDAKAQKLLVGVDEKLNPEDI
KKHLLVHAPEDKKEILASFISGLFNFYEDLYFTYLEINPLVVTKDGV
| | CANQASETAVAKNQALKEAGVFVPRSFDELGEIIQSVYEDLVANGVIVPA
QEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITE
| | YICKVKWGDIEFPPPFGREAYPEEAYIADLDAKSGASLKLTLLNPKGRIW
TMVAGGGASVVYSDTICDLGGVNELANYGEYSGAPSEQQTYDYAKTILSL
M
| | TTSAIQNRFKYARVTPDTDWARLLQDHPWLLSQNLVVKPDQLIKRRGKLG
LVGVNLTLDGVKSWLKPRLGQEATVGKATGFLKNFLIEPF
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Matching transcripts |
ACLY-001 - ENSP00000253792 [100%] ACLY-002 - ENSP00000377474 [100%] ACLY-003 - ENSP00000466259 [100%] ACLY-201 - ENSP00000345398 [100%]
| | ACLY-001 - ENSP00000253792 [100%] ACLY-002 - ENSP00000377474 [100%] ACLY-003 - ENSP00000466259 [100%] ACLY-004 - ENSP00000445349 [100%] ACLY-201 - ENSP00000345398 [100%]
| | ACLY-001 - ENSP00000253792 [100%] ACLY-002 - ENSP00000377474 [100%] ACLY-003 - ENSP00000466259 [100%] ACLY-201 - ENSP00000345398 [100%]
| | ACLY-001 - ENSP00000253792 [100%] ACLY-002 - ENSP00000377474 [100%] ACLY-003 - ENSP00000466259 [100%] ACLY-201 - ENSP00000345398 [100%] ACLY-005 - ENSP00000468705 [99%] ACLY-004 - ENSP00000445349 [82%]
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Other gene match |
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ANTIBODY VALIDATION
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Immunohistochemistry
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Image |
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Description |
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells. More information | | Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islet cells. More information | | Immunohistochemical staining of human pancreas shows strong cytoplasmic and nuclear positivity in islet cells. More information | | Immunohistochemical staining of human oral mucosa shows strong cytoplasmic positivity in squamous epithelial cells. More information | | Immunohistochemical staining of human kidney shows distinct nuclear and cytoplasmic positivity in cells in tubules. More information | |
Retrieval method |
HIER pH6 | | HIER pH6 | | HIER pH6 | | HIER pH6 | | HIER pH6 | |
Antibody dilution |
1:75 | | 1:35 | | 1:90 | | 1:20 | | 1:200 | |
Literature conformity |
Partly consistent with extensive gene/protein characterization data | | Partly consistent with extensive gene/protein characterization data | | Partly consistent with extensive gene/protein characterization data | | Partly consistent with extensive gene/protein characterization data | | Partly consistent with extensive gene/protein characterization data | |
RNA consistency |
Not done | | Not done | | Not done | | Not done | | Not done | |
Immunofluorescence
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Description |
Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & cytoplasm. More information | | Immunofluorescent staining of human cell line U-251 MG shows positivity in plasma membrane & cytoplasm. More information | | Immunofluorescent staining of human cell line U-251 MG shows positivity in plasma membrane, cytoplasm & nucleus but excluded from the nucleoli. More information | | Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm & nucleus but excluded from the nucleoli. More information | | Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm & nucleus but excluded from the nucleoli. More information | |
Antibody dilution |
1:10 | | 1:10 | | 1:20 | | 1:20 | | 1:50 | |
Validation IF |
Supportive: The subcellular location is supported by experimental gene/protein characterization data, gene silencing, or an independent antibody. | | Supportive: The subcellular location is supported by experimental gene/protein characterization data, gene silencing, or an independent antibody. | | Supportive: The subcellular location is supported by experimental gene/protein characterization data, gene silencing, or an independent antibody. | | Supportive: The subcellular location is supported by experimental gene/protein characterization data, gene silencing, or an independent antibody. | | Supportive: The subcellular location is supported by experimental gene/protein characterization data, gene silencing, or an independent antibody. | |
Western Blot
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Image |
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Description |
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil More information | | Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil More information | | Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil More information | | Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil More information | | Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil More information | |
Target mass (kDa) |
120.8, 119.8 | | 120.8, 119.8, 91.1 | | 120.8, 119.8 | | 120.8, 119.8, 91.1, 12.3 | | 120.8, 119.8, 91.1, 14.2, 12.3 | |
Antibody dilution |
1:250 | | 1:250 | | 1:250 | | 1:250 | | 1:500 | |
Validation WB |
Supportive: Single band corresponding to the predicted size in kDa (+/-20%) | | Supportive: Band of predicted size in kDa (+/-20%) with additional bands present | | Supportive: Single band corresponding to the predicted size in kDa (+/-20%) | | Supportive: Band of predicted size in kDa (+/-20%) with additional bands present | | Supportive: Single band corresponding to the predicted size in kDa (+/-20%) | |
Protein array
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Description |
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green. More information | | Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green. More information | | Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green. More information | | Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green. More information | | Application not done for this antibody. | |
Antibody dilution |
1:500 | | 1:500 | | 1:3000 | | 1:3000 | | | |
Validation PA |
Supportive: Pass with single peak corresponding to interaction only with its own antigen. | | Supportive: Pass with single peak corresponding to interaction only with its own antigen. | | Supportive: Pass with single peak corresponding to interaction only with its own antigen. | | Supportive: Pass with single peak corresponding to interaction only with its own antigen. | | | |
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