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GENERAL INFORMATION
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Protein class
Protein class the gene product belongs to according to selected gene lists. List of protein classes.
Predicted localization
Protein localization predicted from several bioinformatic algorithms. More info...
Number of transcripts
Number of protein-coding transcribed from this gene as defined by Ensembl.
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Gene name |
P4HA1 |
Gene description |
Prolyl 4-hydroxylase, alpha polypeptide I |
Protein class |
Enzymes
Plasma proteins
Predicted secreted proteins |
Predicted localization |
Secreted |
Number of transcripts |
5 |
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HUMAN PROTEIN ATLAS INFORMATION
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RNA tissue category
The RNA tissue category is based on mRNA expression levels in 32 different tissues ( RNA assay description).
The categories include: tissue enriched, group enriched, tissue enhanced, expressed in all, not detected, and mixed.
RNA tissue category is calculated separately both for internally generated Human Protein Atlas (HPA) RNA-seq data, as
well as RNA-seq data from the Genotype-Tissue Expression ( GTEx) consortium.
Protein evidence
Protein evidence scores are generated from several independent sources
and are classified as evidence at i) protein level, ii) transcript level, iii) no evidence or iv) not available.
Protein localization
A summary of the overall protein expression pattern across the analyzed tissues. The summary is based on knowledge-based
annotation ( Annotation description). For genes pending
knowledge-based annotation, the protein localization refers to the antibody staining pattern.
"RNA-based expert annotation gave inconconclusive results" is shown for genes analyzed with a knowledge-based approach where
available RNA-seq and gene/protein characterization data has been evaluated as not sufficient in combination with
immunohistochemistry-data to yield a reliable estimation of the protein expression profile.
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RNA tissue category |
| HPA: | Expressed in all |
| GTEx: | Expressed in all |
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Protein evidence |
Evidence at protein level |
Protein localization |
Ubiquitous cytoplasmic expression at variable levels. |
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ANTIBODY RELIABILITY
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Data reliability description
Standardized explanatory sentences with additional information required for full understanding of the
knowledge-based expression profile. Currently, not all genes have been analyzed by knowledge-based
annotation, indicated by "Pending RNA-based expert annotation", when applicable.
Data reliability
Data reliability score ( score description), divided into supportive (premium)
or uncertain (not premium), is based on consistency between the staining pattern of one antibody or several
antibodies with RNA-seq data and available gene/protein characterization data.
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Data reliability
description |
Pending RNA-based expert annotation. |
Data reliability |
Supportive based on 2 antibodies.
HPA007599
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HPA026593
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PROTEIN EXPRESSION OVERVIEW
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Protein expression data is shown for each of the 44 tissues. Color-coding is based on 13 tissue groups, each consisting
of tissues with common functional features. Mouse-over function shows protein score for analyzed cell types found in a
selected tissue. To access image data click on tissue name or bar. Annotation of protein expression is described in
detail in Assays & annotation.
For genes with available protein data for which a knowledge-based annotation could not be performed, no protein
expression data is displayed in the protein expression data overview, however, all immunohistochemical images are
still available and the annotation data can be found under Primary data.
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GENE INFORMATION
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Gene information from Ensembl and Entrez, as well as links to available gene identifiers are displayed here. Information was retrieved from Ensembl if not indicated otherwise.
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Gene name |
P4HA1 (HGNC Symbol) |
Synonyms |
C-P4Halpha(I), P4HA |
Description |
Prolyl 4-hydroxylase, alpha polypeptide I (HGNC Symbol) |
Entrez gene summary |
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
Chromosome |
10 |
Cytoband |
q22.1 |
Chromosome location (bp) |
73007217 - 73096974 |
Protein evidence |
Evidence at protein level (all genes) |
Ensembl |
ENSG00000122884 (version 78.38) |
Entrez gene |
5033 |
UniProt |
P13674 (UniProt - Evidence at protein level) |
neXtProt |
NX_P13674 |
Antibodypedia |
P4HA1 antibodies |
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PROTEIN BROWSER
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The protein browser displays the antigen location on the target protein(s) and the features of the target protein. The
tabs at the top of the protein view section can be used to switch between the different splice variants to which
an antigen has been mapped.
At the top of the view, the position of the antigen (identified by the corresponding HPA identifier) is shown as a green
bar. A yellow triangle on the bar indicates a <100% sequence identity to the protein target.
Under the antigens, the maximum percent sequence identity of the protein to all other proteins from other human genes is
displayed, using a sliding window of 10 aa residues (HsID 10) or 50 aa residues
(HsID 50) ( read more).
If a signal peptide is predicted by a majority of the signal peptide predictors SPOCTOPUS,
SignalP 4.0, and
Phobius (turquoise)
and/or transmembrane regions (orange) are predicted by MDM, these are displayed.
Low complexity regions are shown in yellow
and InterPro regions in green. Common (purple) and
unique (grey) regions between different splice variants of the gene are also displayed
( read more), and at the
bottom of the protein view is the protein scale.
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PROTEIN INFORMATION
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The protein information section displays alternative protein-coding transcripts (splice variants) encoded by this
gene according to the Ensembl database.
The ENSP identifier links to the Ensembl website protein summary, while the ENST identifier links to the Ensembl website transcript summary
for the selected splice variant. The data in the UniProt column can be expanded to show links to all matching
UniProt identifiers for this protein.
The protein classes assigned to this protein are shown if expanding the data in the
protein class column. Parent protein classes are in bold font and subclasses are listed under the parent class.
The Gene Ontology terms assigned to this protein are
listed if expanding the Gene ontology column.
The length of the protein (amino acid residues according to Ensembl), molecular mass (kDalton), predicted signal peptide (according to a
majority of the signal peptide predictors SPOCTOPUS,
SignalP 4.0 and
Phobius) and the number of predicted transmembrane region(s)
(according to MDM) are also reported.
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Splice variant |
Ensembl |
UniProt |
Protein class |
Gene ontology |
Length
(aa) |
Molecular mass
(kDa) |
Signal peptide
(predicted) |
Transmembrane regions
(predicted)
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| P4HA1-001 |
ENSP00000307318
ENST00000307116
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P13674
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P13674 [Direct mapping]Prolyl 4-hydroxylase subunit alpha-1
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Enzymes
ENZYME proteins
Oxidoreductases
SPOCTOPUS predicted membrane proteins
Predicted secreted proteins
Secreted proteins predicted by MDSEC
SignalP predicted secreted proteins
Phobius predicted secreted proteins
Plasma proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
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GO:0004656 [procollagen-proline 4-dioxygenase activity] GO:0005506 [iron ion binding] GO:0005515 [protein binding] GO:0005739 [mitochondrion] GO:0005783 [endoplasmic reticulum] GO:0016020 [membrane] GO:0016491 [oxidoreductase activity] GO:0016702 [oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen] GO:0016705 [oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen] GO:0016706 [oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors] GO:0031418 [L-ascorbic acid binding] GO:0043231 [intracellular membrane-bounded organelle] GO:0055114 [oxidation-reduction process]
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534 |
61 |
Yes |
0 |
| P4HA1-002 |
ENSP00000362099
ENST00000373008
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P13674
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P13674 [Direct mapping]Prolyl 4-hydroxylase subunit alpha-1 Q5VSQ6 [Target identity:100%; Query identity:100%]Procollagen-proline, 2-oxoglutarate 4-dioxygenase (Proline 4-hydroxylase), alpha polypeptide I variant {ECO:0000313|EMBL:BAD96680.1}; Procollagen-proline, 2-oxoglutarate 4-dioxygenase (Proline 4-hydroxylase), alpha polypeptide I, isoform CRA_a {ECO:0000313|EMBL:EAW54467.1}; Procollagen-proline, 2-oxoglutarate 4-dioxygenase (Proline 4-hydroxylase), alpha polypeptide I, isoform CRA_b {ECO:0000313|EMBL:EAW54468.1}
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Enzymes
ENZYME proteins
Oxidoreductases
SPOCTOPUS predicted membrane proteins
Predicted secreted proteins
Secreted proteins predicted by MDSEC
SignalP predicted secreted proteins
Phobius predicted secreted proteins
Plasma proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
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GO:0004656 [procollagen-proline 4-dioxygenase activity] GO:0005506 [iron ion binding] GO:0005515 [protein binding] GO:0005739 [mitochondrion] GO:0005783 [endoplasmic reticulum] GO:0016020 [membrane] GO:0016491 [oxidoreductase activity] GO:0016702 [oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen] GO:0016705 [oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen] GO:0016706 [oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors] GO:0031418 [L-ascorbic acid binding] GO:0043231 [intracellular membrane-bounded organelle] GO:0055114 [oxidation-reduction process]
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534 |
61 |
Yes |
0 |
| P4HA1-201 |
ENSP00000263556
ENST00000263556
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P13674
Show all »
« Hide
P13674 [Direct mapping]Prolyl 4-hydroxylase subunit alpha-1 Q5VSQ6 [Target identity:100%; Query identity:100%]Procollagen-proline, 2-oxoglutarate 4-dioxygenase (Proline 4-hydroxylase), alpha polypeptide I variant {ECO:0000313|EMBL:BAD96680.1}; Procollagen-proline, 2-oxoglutarate 4-dioxygenase (Proline 4-hydroxylase), alpha polypeptide I, isoform CRA_a {ECO:0000313|EMBL:EAW54467.1}; Procollagen-proline, 2-oxoglutarate 4-dioxygenase (Proline 4-hydroxylase), alpha polypeptide I, isoform CRA_b {ECO:0000313|EMBL:EAW54468.1}
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Show »
« Hide
Enzymes
ENZYME proteins
Oxidoreductases
SPOCTOPUS predicted membrane proteins
Predicted secreted proteins
Secreted proteins predicted by MDSEC
SignalP predicted secreted proteins
Phobius predicted secreted proteins
Plasma proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
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Show »
« Hide
GO:0004656 [procollagen-proline 4-dioxygenase activity] GO:0005506 [iron ion binding] GO:0005515 [protein binding] GO:0005739 [mitochondrion] GO:0005783 [endoplasmic reticulum] GO:0016020 [membrane] GO:0016222 [procollagen-proline 4-dioxygenase complex] GO:0016491 [oxidoreductase activity] GO:0016702 [oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen] GO:0016705 [oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen] GO:0016706 [oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors] GO:0018401 [peptidyl-proline hydroxylation to 4-hydroxy-L-proline] GO:0030199 [collagen fibril organization] GO:0031418 [L-ascorbic acid binding] GO:0043231 [intracellular membrane-bounded organelle] GO:0055114 [oxidation-reduction process]
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534 |
61 |
Yes |
0 |
| P4HA1-202 |
ENSP00000378353
ENST00000394890
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P13674
Show all »
« Hide
P13674 [Direct mapping]Prolyl 4-hydroxylase subunit alpha-1
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Show »
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Enzymes
ENZYME proteins
Oxidoreductases
SPOCTOPUS predicted membrane proteins
Predicted secreted proteins
Secreted proteins predicted by MDSEC
SignalP predicted secreted proteins
Phobius predicted secreted proteins
Plasma proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
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Show »
« Hide
GO:0004656 [procollagen-proline 4-dioxygenase activity] GO:0005506 [iron ion binding] GO:0005515 [protein binding] GO:0005739 [mitochondrion] GO:0005783 [endoplasmic reticulum] GO:0016020 [membrane] GO:0016491 [oxidoreductase activity] GO:0016702 [oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen] GO:0016705 [oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen] GO:0016706 [oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors] GO:0031418 [L-ascorbic acid binding] GO:0043231 [intracellular membrane-bounded organelle] GO:0055114 [oxidation-reduction process]
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534 |
61 |
Yes |
0 |
| P4HA1-203 |
ENSP00000414464
ENST00000440381
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P13674
Show all »
« Hide
P13674 [Direct mapping]Prolyl 4-hydroxylase subunit alpha-1
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Show »
« Hide
Enzymes
ENZYME proteins
Oxidoreductases
SPOCTOPUS predicted membrane proteins
Predicted secreted proteins
Secreted proteins predicted by MDSEC
SignalP predicted secreted proteins
Phobius predicted secreted proteins
Plasma proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
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Show »
« Hide
GO:0004656 [procollagen-proline 4-dioxygenase activity] GO:0005506 [iron ion binding] GO:0005515 [protein binding] GO:0005739 [mitochondrion] GO:0005783 [endoplasmic reticulum] GO:0016020 [membrane] GO:0016491 [oxidoreductase activity] GO:0016702 [oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen] GO:0016705 [oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen] GO:0016706 [oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors] GO:0031418 [L-ascorbic acid binding] GO:0043231 [intracellular membrane-bounded organelle] GO:0055114 [oxidation-reduction process]
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516 |
59 |
Yes |
0 |
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